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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/06/2023 |
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Actualizado : |
21/06/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RODRIGUEZ, N.E.; BEYHAUT, E.; SICARDI, M.; RODRÍGUEZ-BLANCO, A. |
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Afiliación : |
N. E. RODRIGUEZ RODRIGUEZ, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. SICARDI, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; A. RODRÍGUEZ-BLANCO, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Bioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils: a genetic and symbiotic approach. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Environmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1 |
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ISSN : |
2523-8922 (electronic). |
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DOI : |
10.1007/s42398-022-00258-1 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy -- |
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Contenido : |
ABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023. MenosABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots,... Presentar Todo |
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Palabras claves : |
Biological nitrogen fixation; Bradyrhizobium; Glycine max; MLSA; Phylogeny; PLATAFORMA DE BIOINSUMOS - INIA; Rhizobia. |
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Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
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Marc : |
LEADER 03065naa a2200277 a 4500
001 1064205
005 2023-06-21
008 2023 bl uuuu u00u1 u #d
022 $a2523-8922 (electronic).
024 7 $a10.1007/s42398-022-00258-1$2DOI
100 1 $aRODRIGUEZ, N.E.
245 $aBioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils$ba genetic and symbiotic approach.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy --
520 $aABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023.
653 $aBiological nitrogen fixation
653 $aBradyrhizobium
653 $aGlycine max
653 $aMLSA
653 $aPhylogeny
653 $aPLATAFORMA DE BIOINSUMOS - INIA
653 $aRhizobia
700 1 $aBEYHAUT, E.
700 1 $aSICARDI, M.
700 1 $aRODRÍGUEZ-BLANCO, A.
773 $tEnvironmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1
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